Evaluation of target specificity of antibacterial agents using Staphylococcus aureus ddlA mutants and D-cycloserine in a silkworm infection model.

نویسندگان

  • Kenji Kurokawa
  • Hiroshi Hamamoto
  • Miki Matsuo
  • Satoshi Nishida
  • Noriko Yamane
  • Bok Luel Lee
  • Kazuhisa Murakami
  • Hideki Maki
  • Kazuhisa Sekimizu
چکیده

The availability of a silkworm larva infection model to evaluate the therapeutic effectiveness of antibiotics was examined. The 50% effective doses (ED50) of D-cycloserine against the Staphylococcus aureus ddlA mutant-mediated killing of larvae were remarkably lower than those against the parental strain-mediated killing of larvae. Changes in MICs and ED50 of other antibiotics were negligible, suggesting that these alterations are d-cycloserine selective. Therefore, this model is useful for selecting desired compounds based on their therapeutic effectiveness during antibiotic development.

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عنوان ژورنال:
  • Antimicrobial agents and chemotherapy

دوره 53 9  شماره 

صفحات  -

تاریخ انتشار 2009